ALL STAR CHEER SQUAD for the Wii is a aerobic game which teaches the player traditional cheerleading moves and combines it with dance, gymnastics, and squad-based teamwork to create a fun, challenging game. The Fox cheerleading squad is short a member when one of their cheerleaders suffers an ankle injury. Fresh out of Cheer Camp, you are ready for action and join the squad. As a rhythm-based game, icons of how to position the remote and nunchuk move across the screen from right to left along a "cheer line." The object of the game is to mimic the positions as they enter a "hit area" marked by a circle and star. Like other games of the genre, if you match the required movements at the right time, you score the most possible points.
Players start off by creating and naming an avatar, choosing gender, skin tone, facial features, outfits, and even make-up. Then you are taken through practice sessions where voice-over as well as text instructions are provided, teaching you new movements. Practice sessions can be played repeatedly until a pass grade of a C is earned to unlock new cheer moves. Players then move on to challenges and competitions, including a "versus" game mode where players attack, charge, and defend their cheer line using cheer movements.